Apoptotic effects of hyperoside on FaDu human pharyngeal carcinoma cells
강경록, 서정연, Lim HyangI, 김재성, 김도경,
소속 상세정보
강경록 ( Kang Kyeong-Rok ) - Chosun University Institute of Dental Science
서정연 ( Seo Jeong-Yeon ) -
( Lim HyangI ) - Chosun University Institute of Dental Science
김재성 ( Kim Jae-Sung ) - Chosun University Institute of Dental Science
김도경 ( Kim Do-Kyung ) -
Abstract
Hyperoside (quercetin 3-o-β-d-galactopyranoside) is a flavonoid glycoside with antidepressant, anti-inflammatory, and anti-cancer effects. However, it is not known whether it is effective for oral cancer. This study aimed to investigate the effects of hyperoside on induction of apoptosis in FaDu human pharyngeal carcinoma cells. It employed 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, live/dead staining, 4′,6-diamidino-2-phenylindole (DAPI) staining, hematoxylin and eosin staining, and western blotting to analyze FaDu cells. The MTT assay, live/dead staining, and DAPI staining showed that hyperoside increased FaDu cell apoptosis in a concentration-dependent manner. Hyperoside-induced apoptosis in FaDu cells was mediated by the expression of Fas and activation of caspase-8, caspase-3, and poly (ADP-ribose) polymerase. Western blotting results showed that Bcl-2 and Bcl-xL were downregulated but Bad and Bax were upregulated by hyperoside in FaDu cells. These results suggest that hyperoside inhibits cell proliferation in FaDu human pharyngeal carcinoma cells and induces apoptosis through receptor-mediated extrinsic apoptosis and mitochondrial-mediated endogenous apoptosis pathways.
키워드
Apoptosis; FaDu; Hyperoside; Oral cancer
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